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length sars cov 2 spike  (Sino Biological)


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    Structured Review

    Sino Biological length sars cov 2 spike
    Length Sars Cov 2 Spike, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+human+codon+optimized+spike+gene/Human+SARS+coronavirus+(SARS-CoV)+Spike+Gene+ORF+cDNA+clone+expression+plasmid(Codon+Optimized)%2C+C-His+tag/10__7554_slash_elife__99833__3-460-10-23
    Average 94 stars, based on 3 article reviews
    length sars cov 2 spike - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: A single dose of recombinant VSV-ΔG-spike vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: .. The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers: Forward – atcgatctgtttacgcgtcactATGTTTGTGTTCCTGGTGCTGC; Reverse – atgaagaatctggctagcaggatttgagTCAGGTGTAGTGCAGTTTCACTCC. .. The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene.

    Plasmid Preparation:

    Article Title: A single dose of recombinant VSV-ΔG-spike vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: .. The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers: Forward – atcgatctgtttacgcgtcactATGTTTGTGTTCCTGGTGCTGC; Reverse – atgaagaatctggctagcaggatttgagTCAGGTGTAGTGCAGTTTCACTCC. .. The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene.

    Construct:

    Article Title: A single dose of recombinant VSV-ΔG-spike vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: .. The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers: Forward – atcgatctgtttacgcgtcactATGTTTGTGTTCCTGGTGCTGC; Reverse – atgaagaatctggctagcaggatttgagTCAGGTGTAGTGCAGTTTCACTCC. .. The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene.

    Polymerase Chain Reaction:

    Article Title: A single dose of recombinant VSV-ΔG-spike vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: .. The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers: Forward – atcgatctgtttacgcgtcactATGTTTGTGTTCCTGGTGCTGC; Reverse – atgaagaatctggctagcaggatttgagTCAGGTGTAGTGCAGTTTCACTCC. .. The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene.

    Amplification:

    Article Title: A single dose of recombinant VSV-ΔG-spike vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: .. The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers: Forward – atcgatctgtttacgcgtcactATGTTTGTGTTCCTGGTGCTGC; Reverse – atgaagaatctggctagcaggatttgagTCAGGTGTAGTGCAGTTTCACTCC. .. The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene.



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